Bd Biosciences Cst Bead Lot Files
Bd Biosciences Cst Bead Lot Files Online
Name Bead Lot Files Status Part Number; BD™ CS&T. When running a BD FACSLyric™ 12-color IVD instrument, we recommend using lot 9051880 or newer. This new lot of beads, and all future lots, have been optimized for the 12-color instrument. After saving the bead lot file in the proper folder, unzip it and start BD FACSDiva software on the cytometer workstation. Within BD FACSDiva software, select Cytometer CST Tools Bead Lots. In the Bead Lot window, click the Import button. Select the appropriate bead lot file. Detector Performance, BD Biosciences Application Note, June 2012. Hoffman, Resolution of dimly fluorescent particles: a practical measure of fluorescence sensitivity. Flow Cytometry H4IIE hepatocytes were grown to confluence in 6-well plates (Corning Costar® Cat# 3516) and then treated with/without recombinant CTRP3-FLAG as indicated.The cells were then collected in PBS, fixed in 4% formaldehyde, and then incubated with rabbit anti-FLAG antibody (Cell Signaling Technology, Cat# 14793; RRID: AB2572291. The purpose of this first section is to review the current instrument set up procedure recommended by BD Biosciences(7,8). Cytometer Setup-Tracking beads (CST) are a set of 3 uniformly stained fluorescent beads.